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sirna delivery  (Mirus Bio)


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    Structured Review

    Mirus Bio sirna delivery
    Sirna Delivery, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 98/100, based on 2310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirna+delivery/TransIT-X2/pmc12993322-317-1-11
    Average 98 stars, based on 2310 article reviews
    sirna delivery - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Transfection:

    Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity.
    Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods.

    Article Title: Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems
    Article Snippet: .. N 4 -myristoleoyl- N 9 -myristoylspermine ( 6c ) and N 4 -oleoyl- N 9 -stearoylspermine ( 6d ) showed the highest efficiency of siRNA delivery into FEK4 and HtTA cells, comparable to the efficiency of the commercial transfectant TransIT-TKO (Mirus Bio, Madison, WI, USA). ..

    Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters.
    Article Snippet: .. Peptide length was set to 7–30, precursor range 350–1,650, and fragment range 300–2,000, and the FDR was set to 1%. siRNA-mediated gene knockdown in adipocytes For siRNA delivery, 3T3-L1 adipocytes were reverse transfected using the TransIT-X2 (Mirus, Cat. #MIR6006) as previously described (12, 109). .. Briefly, siRNA targeting mouse SLC20A2 (M-062508-01; siGENOME SMARTpool; Dharmacon Horizons), SLC12A4 (M-044424-01; Jo urn l P re- pro of 24 siGENOME SMARTpool; Dharmacon Horizons) or non-targeting control siRNA (D-00120613; siGENOME SMARTpool; Dharmacon Horizons) was added to an Opti-MEM/TransIT-X2 mix (final concentration of siRNA added to cells = 50 nM), and incubated at RT for 30 min. Resuspended adipocytes in DMEM/FBS/GlutaMAX were added to the OptiMEM/TransITX2/siRNA mixes and immediately seeded into Matrigel-coated 96- (PerkinElmer Cell Carrier Ultra, Cat. #6055300) or 48-well plates for assessing GLUT4 translocation or 2-deoxyglucose uptake, respectively.

    Article Title: Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.
    Article Snippet: Specific siRNA for prothymosin α was purchased from Sigma. .. For siRNA delivery, cells were incubated for 24 h (as indicated by preliminary experiments performed at 12, 24 or 48 h) at 37 °C in 5% CO2 with specific siRNA using TransIT-TKO Transfection Reagent (Mirus) following the manufacturer’s instructions. ..

    Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters
    Article Snippet: .. For siRNA delivery, 3T3-L1 adipocytes were reverse transfected using the TransIT-X2 (Mirus, Cat. #MIR6006) as previously described ( , ). .. Briefly, siRNA targeting mouse SLC20A2 (M-062508–01; siGENOME SMARTpool; Dharmacon Horizons), SLC12A4 (M-044424-01; siGENOME SMARTpool; Dharmacon Horizons) or nontargeting control siRNA (D-001206-13; siGENOME SMARTpool; Dharmacon Horizons) was added to an Opti-MEM/TransIT-X2 mix (final concentration of siRNA added to cells = 50 nM), and incubated at RT for 30 min. Resuspended adipocytes in DMEM/FBS/GlutaMAX were added to the OptiMEM/TransIT-X2/siRNA mixes and immediately seeded into Matrigel-coated 96- (PerkinElmer Cell Carrier Ultra, Cat. #6055300) or 48-well plates for assessing GLUT4 translocation or 2-deoxyglucose uptake, respectively.

    Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells.
    Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. siRNA delivery and knockdown of COUP-TFII was obtained by TransIT-TKO Transfection Reagent 8 (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Specific and validate siRNA were purchased from QIAGEN (QIAGEN S.p.A, Milan Italy).

    In Vitro:

    Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity.
    Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods.

    Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery.
    Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized.

    Transduction:

    Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity.
    Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods.

    Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery.
    Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized.

    MTT Assay:

    Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery.
    Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized.

    Control:

    Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery.
    Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized.

    Knockdown:

    Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters.
    Article Snippet: .. Peptide length was set to 7–30, precursor range 350–1,650, and fragment range 300–2,000, and the FDR was set to 1%. siRNA-mediated gene knockdown in adipocytes For siRNA delivery, 3T3-L1 adipocytes were reverse transfected using the TransIT-X2 (Mirus, Cat. #MIR6006) as previously described (12, 109). .. Briefly, siRNA targeting mouse SLC20A2 (M-062508-01; siGENOME SMARTpool; Dharmacon Horizons), SLC12A4 (M-044424-01; Jo urn l P re- pro of 24 siGENOME SMARTpool; Dharmacon Horizons) or non-targeting control siRNA (D-00120613; siGENOME SMARTpool; Dharmacon Horizons) was added to an Opti-MEM/TransIT-X2 mix (final concentration of siRNA added to cells = 50 nM), and incubated at RT for 30 min. Resuspended adipocytes in DMEM/FBS/GlutaMAX were added to the OptiMEM/TransITX2/siRNA mixes and immediately seeded into Matrigel-coated 96- (PerkinElmer Cell Carrier Ultra, Cat. #6055300) or 48-well plates for assessing GLUT4 translocation or 2-deoxyglucose uptake, respectively.

    Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells.
    Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. siRNA delivery and knockdown of COUP-TFII was obtained by TransIT-TKO Transfection Reagent 8 (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Specific and validate siRNA were purchased from QIAGEN (QIAGEN S.p.A, Milan Italy).

    Incubation:

    Article Title: Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis.
    Article Snippet: Specific siRNA for prothymosin α was purchased from Sigma. .. For siRNA delivery, cells were incubated for 24 h (as indicated by preliminary experiments performed at 12, 24 or 48 h) at 37 °C in 5% CO2 with specific siRNA using TransIT-TKO Transfection Reagent (Mirus) following the manufacturer’s instructions. ..

    Luciferase:

    Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells.
    Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. siRNA delivery and knockdown of COUP-TFII was obtained by TransIT-TKO Transfection Reagent 8 (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Specific and validate siRNA were purchased from QIAGEN (QIAGEN S.p.A, Milan Italy).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells.
    Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. siRNA delivery and knockdown of COUP-TFII was obtained by TransIT-TKO Transfection Reagent 8 (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Specific and validate siRNA were purchased from QIAGEN (QIAGEN S.p.A, Milan Italy).



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    Image Search Results


    Variations in Nanoparticle Type Affecting the CNS. The major classes of nanoparticles are shown in Fig. 2 with the significant parameter for CNS targeting delivery due to biological barriers identified, including surface functionalization with targeting ligands (e.g., transferrin, ApoE peptides); PEGylation to prolong circulation time; optimization of size for enhanced transcytosis; and schematic representation of pathways for cellular uptake and intracellular trafficking of nucleic acids. These engineered characteristics are important for overcoming biological barriers and improving gene expression in neurons and glial cells. In particular, lipid-based delivery systems have demonstrated strong translational potential, owing to their use as mRNA platforms for vaccines and their modifiability to deliver RNA and CRISPRs. (Zhu et al. )

    Journal: Cellular and Molecular Neurobiology

    Article Title: Recent Advances in the Non-viral Delivery of Genes to Central Nervous System Disorders

    doi: 10.1007/s10571-026-01703-z

    Figure Lengend Snippet: Variations in Nanoparticle Type Affecting the CNS. The major classes of nanoparticles are shown in Fig. 2 with the significant parameter for CNS targeting delivery due to biological barriers identified, including surface functionalization with targeting ligands (e.g., transferrin, ApoE peptides); PEGylation to prolong circulation time; optimization of size for enhanced transcytosis; and schematic representation of pathways for cellular uptake and intracellular trafficking of nucleic acids. These engineered characteristics are important for overcoming biological barriers and improving gene expression in neurons and glial cells. In particular, lipid-based delivery systems have demonstrated strong translational potential, owing to their use as mRNA platforms for vaccines and their modifiability to deliver RNA and CRISPRs. (Zhu et al. )

    Article Snippet: Representative Applications in CNS , Nanoparticle DNA/siRNA delivery for neurodegeneration , LNP-mediated mRNA/siRNA delivery; CRISPR mRNA/RNP CNS editing , Exosome delivery of RNA/proteins; CRISPR cargo delivery , Exosome/LNP delivery of CRISPR to HD, AD, and PD models.

    Techniques: Gene Expression, Vaccines

    Genome Editing Delivery Systems. Design Features for a Nanoparticle-Based Delivery System with CRISPR-Cas9 Genome Editing Technology. This article depicts a multi-functional, non-viral delivery system designed specifically for CRISPR-Cas9 genome editing. An illustration is presented that highlights key elements of the design model, such as the incorporation of an activatable protective layer to prevent degradation of Cas9 during circulation; the use of positively charged polymer materials that can facilitate nucleic acid complexation and promote rapid and efficient endosomal escape; the addition of a biocompatible outer layer to decrease the likelihood of nonspecific protein binding; techniques used to target specific areas of the body leading to improved delivery efficiency and enhanced transcytosis of the nanoparticles through biological barriers, characterizing the additions of antigenic motifs to reduce the immune response and establish long-lived systemic stability for the delivery nanoparticles. (Tong et al. )

    Journal: Cellular and Molecular Neurobiology

    Article Title: Recent Advances in the Non-viral Delivery of Genes to Central Nervous System Disorders

    doi: 10.1007/s10571-026-01703-z

    Figure Lengend Snippet: Genome Editing Delivery Systems. Design Features for a Nanoparticle-Based Delivery System with CRISPR-Cas9 Genome Editing Technology. This article depicts a multi-functional, non-viral delivery system designed specifically for CRISPR-Cas9 genome editing. An illustration is presented that highlights key elements of the design model, such as the incorporation of an activatable protective layer to prevent degradation of Cas9 during circulation; the use of positively charged polymer materials that can facilitate nucleic acid complexation and promote rapid and efficient endosomal escape; the addition of a biocompatible outer layer to decrease the likelihood of nonspecific protein binding; techniques used to target specific areas of the body leading to improved delivery efficiency and enhanced transcytosis of the nanoparticles through biological barriers, characterizing the additions of antigenic motifs to reduce the immune response and establish long-lived systemic stability for the delivery nanoparticles. (Tong et al. )

    Article Snippet: Representative Applications in CNS , Nanoparticle DNA/siRNA delivery for neurodegeneration , LNP-mediated mRNA/siRNA delivery; CRISPR mRNA/RNP CNS editing , Exosome delivery of RNA/proteins; CRISPR cargo delivery , Exosome/LNP delivery of CRISPR to HD, AD, and PD models.

    Techniques: CRISPR, Functional Assay, Polymer, Protein Binding

    Translocation across the blood-brain barrier. Figure shows the main ways in which engineered gene carriers cross the BBB. These are receptor-mediated transcytosis (RMT) or using receptors like transferrin or apolipoprotein E (ApoE); carrier-mediated transport with ligand conjugated nanoparticles; and adsorptive-mediated transcytosis, where the surface charge of the nanoparticle can affect how easily it will cross. There are also peptide-mediated transport methods, such as those using TAT or rabies virus glycoprotein (RVG) peptides, developed to promote cell uptake and BBB crossing. Physical methods, such as focused ultrasound (FUS) inducing temporary BBB disruption will increase the amount delivered by increasing the permeability of the BBB. The figure summarizes transport strategies telling how surface ligand modifications, optimize size of nanoparticles (especially less than 100 nm), and external physical modulation together maximize penetration of CNS (central nervous system). To provide for the rational design of gene carriers that optimize balance of efficiency and safety will require a good understanding of all pathways available. (Brookes et al. ; Nguyen et al. )

    Journal: Cellular and Molecular Neurobiology

    Article Title: Recent Advances in the Non-viral Delivery of Genes to Central Nervous System Disorders

    doi: 10.1007/s10571-026-01703-z

    Figure Lengend Snippet: Translocation across the blood-brain barrier. Figure shows the main ways in which engineered gene carriers cross the BBB. These are receptor-mediated transcytosis (RMT) or using receptors like transferrin or apolipoprotein E (ApoE); carrier-mediated transport with ligand conjugated nanoparticles; and adsorptive-mediated transcytosis, where the surface charge of the nanoparticle can affect how easily it will cross. There are also peptide-mediated transport methods, such as those using TAT or rabies virus glycoprotein (RVG) peptides, developed to promote cell uptake and BBB crossing. Physical methods, such as focused ultrasound (FUS) inducing temporary BBB disruption will increase the amount delivered by increasing the permeability of the BBB. The figure summarizes transport strategies telling how surface ligand modifications, optimize size of nanoparticles (especially less than 100 nm), and external physical modulation together maximize penetration of CNS (central nervous system). To provide for the rational design of gene carriers that optimize balance of efficiency and safety will require a good understanding of all pathways available. (Brookes et al. ; Nguyen et al. )

    Article Snippet: Representative Applications in CNS , Nanoparticle DNA/siRNA delivery for neurodegeneration , LNP-mediated mRNA/siRNA delivery; CRISPR mRNA/RNP CNS editing , Exosome delivery of RNA/proteins; CRISPR cargo delivery , Exosome/LNP delivery of CRISPR to HD, AD, and PD models.

    Techniques: Translocation Assay, Virus, Disruption, Permeability

    Non-Viral CNS Gene Delivery Systems. A schematic diagram of various non-viral gene delivery systems under investigation for use in the CNS is shown in Fig. 1. This diagram illustrates that there are a lot of different types of non-viral gene delivery systems, with many different types of devices that can carry DNA, mRNA, siRNA or CRISPR components. In addition, none of these non-viral gene delivery systems have as much immunogenicity, or can be modulated with surface functionality, or can be manufactured on a larger scale as compared with AAV-based gene delivery systems. Non-viral gene delivery systems that were not studied extensively prior to now are classified as “Emerging Systems,” whereas those that are widely studied as lipid or polymer carriers have substantial preclinical evidence before being used in humans

    Journal: Cellular and Molecular Neurobiology

    Article Title: Recent Advances in the Non-viral Delivery of Genes to Central Nervous System Disorders

    doi: 10.1007/s10571-026-01703-z

    Figure Lengend Snippet: Non-Viral CNS Gene Delivery Systems. A schematic diagram of various non-viral gene delivery systems under investigation for use in the CNS is shown in Fig. 1. This diagram illustrates that there are a lot of different types of non-viral gene delivery systems, with many different types of devices that can carry DNA, mRNA, siRNA or CRISPR components. In addition, none of these non-viral gene delivery systems have as much immunogenicity, or can be modulated with surface functionality, or can be manufactured on a larger scale as compared with AAV-based gene delivery systems. Non-viral gene delivery systems that were not studied extensively prior to now are classified as “Emerging Systems,” whereas those that are widely studied as lipid or polymer carriers have substantial preclinical evidence before being used in humans

    Article Snippet: Representative Applications in CNS , Nanoparticle DNA/siRNA delivery for neurodegeneration , LNP-mediated mRNA/siRNA delivery; CRISPR mRNA/RNP CNS editing , Exosome delivery of RNA/proteins; CRISPR cargo delivery , Exosome/LNP delivery of CRISPR to HD, AD, and PD models.

    Techniques: CRISPR, Immunopeptidomics, Polymer