sirna delivery (Mirus Bio)
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Sirna Delivery, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 98/100, based on 2310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirna+delivery/TransIT-X2/pmc12993322-317-1-11
Average 98 stars, based on 2310 article reviews
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Transfection:Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity. Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods. Article Title: Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems Article Snippet: .. N 4 -myristoleoyl- N 9 -myristoylspermine ( 6c ) and N 4 -oleoyl- N 9 -stearoylspermine ( 6d ) showed the highest efficiency of Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters. Article Snippet: .. Peptide length was set to 7–30, precursor range 350–1,650, and fragment range 300–2,000, and the FDR was set to 1%. siRNA-mediated gene knockdown in adipocytes For Article Title: Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis. Article Snippet: Specific siRNA for prothymosin α was purchased from Sigma. .. For Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters Article Snippet: .. For Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells. Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. In Vitro:Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity. Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods. Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery. Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized. Transduction:Article Title: Design and synthesis of N4,N9-disubstituted spermines for non-viral siRNA delivery--structure-activity relationship studies of siFection efficiency versus toxicity. Article Snippet: Purpose.. To study the effect of sequentially changing the chain length, oxidation level, and charge distribution in N,N-diacyl and N,N-dialkyl spermines on siRNA formulation, and then to compare their lipoplex transfection efficiency in cell lines.. Methods. Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery. Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized. MTT Assay:Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery. Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized. Control:Article Title: Very long chain N4, N9 -diacyl spermines: non-viral lipopolyamine vectors for efficient plasmid DNA and siRNA delivery. Article Snippet: Purpose.. To study the effect of increasing the chain length over C-18 and varying the oxidation level in synthesized N,N-diacyl spermines on DNA and siRNA formulation, and then to compare their transfection efficiency in cell lines Methods.. The five novel very long chain N,N-diacyl polyamines: N,N-[diarachidoyl, diarachidonoyl, dieicosenoyl, dierucoyl and dinervonoyl]-1,12-diamino-4,9-diazadodecane were synthesized. Knockdown:Article Title: Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters. Article Snippet: .. Peptide length was set to 7–30, precursor range 350–1,650, and fragment range 300–2,000, and the FDR was set to 1%. siRNA-mediated gene knockdown in adipocytes For Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells. Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. Incubation:Article Title: Thymosin α1 represents a potential potent single-molecule-based therapy for cystic fibrosis. Article Snippet: Specific siRNA for prothymosin α was purchased from Sigma. .. For Luciferase:Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells. Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. Chloramphenicol Acetyltransferase Assay:Article Title: The orphan nuclear receptor COUP-TFII coordinates hypoxia-independent proangiogenic responses in hepatic stellate cells. Article Snippet: DNA vectors and transfection of cultured human HSC Quiescent HSC were transfected with reporter and expression plasmids at the density of 6 x 105 cells/well by TransIT-2020 transfection reagent (Mirus Bio LLC, Medison WI) following the manufacturer's instructions. .. Forty-eight hours after transfection cells were harvested for luciferase and CAT assay as previoulsly described (8), or used for proliferation and invasion assays. |

